Question

Which of the following activities are associated with TFIIH

A it is a kinase that binds to the TATA box

B. it is a helicase and a kinase

C. it is a helicase that is involved in capping of mRNAs

D. none of the above are correct.

E. more than one of the above is correct.

Which of the following is important for sigma factor binding?

A. sequence at -10 box

B. sequence at -35 box

C. spacing between -10 and -35 box

D. A and B only

E. A-C.

What do general transcription factors do?

A. Attach RNA polymerase to the promoter

B. Change the promoter sequence in response to changing environmental conditions

C. Bind to enhancer sequences

D. Recruit chromosome remodeling enzymes

E. Bind to the -10 and -35 boxes of the promoter

Which of the following statement is INCORRECT about TATA binding protein?

A TBP is a subunit of TFIID.

B. TBP recognizes TATA box located upstream of the transcription start site.

C. TATA box needs to be dissociated from histones before TBP binds.

D. TBP recognizes TATA box at the major groove of DNA.

E. The binding of TBP directs assembly of the preinitiation complex.

Conversion of RNA polymerase II from an initiation-specific form to an elongation-specific

form involves

A. phosphorylation of Gal4.

B. phosphorylation of the CTD domain of RNA polymerase II.

C. phosphorylation of the IIb form of RNA polymerase II.

D. phosphorylation of TBP.

Which subunit of prokaryotic RNAP interacts with transcription activators?

A. alpha

B. beta

C. sigma

D. omega

E. more than one of the above

Which of the following is not a major difference between prokaryotic and eukaryotic transcriptional regulatory proteins is…

A. prokaryotic protein binding sites typically overlap the promoters and eukaryotic sites do not.

B. prokaryotes only use transcription activators not inhibitors, but eukaryotes use both.

C. eukaryotic regulator sequences can be near or far from the promoter, but prokaryotic regulatory sequences are typically very close to the promoters.

D. Eukaryotes typically use many more regulatory proteins for each gene than prokaryotes

E. all are reasonable differences

The trp operon is involved in tryptophan synthesis. When tryptophan is present in the environment of E. coli, the tryptophan binds to the

A. trp operon

B. trp promoter

C. trp operator

D. trp repressor

E. trp polymerase

Under conditions of high glucose and high lactose which of the following would be found at the lac operon.

A. lac repressor alone is bound

B. both the lac repressor and CAP/cAMP are bound

C. CAP/cAMP alone is bound

D. neither the lac repressor nor CAP/cAMP are bound.

The 5’ cap is best described as.

A. a 7 methyl G cap going in the same direction as the rest of the nucleotides in the RNA

B. a 7 methyl G cap going in the opposite direction as the rest of the nucleotides in the RNA

C. a 9 methyl G cap going in the opposite direction as the rest of the nucleotides in the RNA

D. an adomet group added to the 5’ end of the RNA.

Exon definition

A. refers to that really cut look that genes get when they work out regularly.

B. refers to a process in which sequences are recognized and used to define the areas of a pre-mRNA that will be included in the mature mRNA.

C. refers to a splicing mechanism that does not involve branch points, nor snRNAs U1, U2, U5 or U6.

D. refers to a process in which sequences are recognized and used to define the areas of a pre-mRNA that will be excluded from the mature mRNA.

Which of the following is the best description of the poly-A signal sequence in a gene?

A. It is a series of T nucleotides that encodes the poly-A tail at the 3' end of the mRNA.

B. It codes for a sequence in the RNA that signals enzymatic cleavage of the RNA several nucleotides away from the sequence.

C. It allows the 3' end of the gene to attach to the ribosome.

D. It is a sequence that codes for binding RNA polymerase to the gene.

Answer 1

activities are associated with TFIIH- B. it is a helicase and a kinase

D. A and B only is important for sigma factor binding

general transcription factors- A. Attach RNA polymerase to the promoter

INCORRECT about TATA binding protein- E. The binding of TBP directs assembly of the preinitiation complex.

Conversion of RNA polymerase II from an initiation-specific form to an elongation-specific form involves - B. phosphorylation of the CTD domain of RNA polymerase II.

The trp operon is involved in tryptophan synthesis. When tryptophan is present in the environment of E. coli, the tryptophan binds to the- D. trp repressor

Under conditions of high glucose and high lactose which of the following would be found at the lac operon- D. neither the lac repressor nor CAP/cAMP are bound.

the best description of the poly-A signal sequence in a gene- B. It codes for a sequence in the RNA that signals enzymatic cleavage of the RNA several nucleotides away from the sequence