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Question 1: You set up a 200 µL PCR to amplify a 1-kb fragment by adding...

Question 1: You set up a 200 µL PCR to amplify a 1-kb fragment by adding 20 µL of a 500 nM primer that is 20-bp in length. How many times would you expect to find the exact 20-bp sequence in the human genome (using a genome size of 3.0 E+9 bp) at random? How many moles of the primer have you added? How many molecules of that primer have you added? Describe whether this amount of primer is a limiting factor in the success of the PCR in relation to the number of copies generated before a PCR normally plateaus (4 marks; show unit conversions and calculations for full marks).

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gladiator answered 2 years ago
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