You want to use PCR to amplify a 1Kb exon of the human autosomal gene phenylalanine...

You want to use PCR to amplify a 1Kb exon of the human autosomal gene phenylalanine hydroxylase (PH) from the genome of a person suffering from the disease phenylketonuria, which is due to this enzyme not functioning.

a) You start with 1 nanogram of genomic DNA in your sample tube. Assuming a human haploid genome is 3 x 109 bp, and each bp has a mass of 660 g/mole, how many moles of haploid genomes are present at the start of your PCR experiment?

b) The PH gene is present in a single copy in the haploid genome. How many template molecules of the PH gene are present at the start of your PCR experiment? (Give your answer in molecules, not moles of molecules.)

c) You set your PCR machine to run for 25 cycles to amplify your 1 kb fragment. Assume every cycle exactly doubles your desired target DNA. At the end of your 25 cycles, how much amplified DNA have you created, in terms of number of molecules AND mass of DNA?

Expert Solution

Stepwise calculations are given below. One thing to remember in such problems is after n th cycle of PCR the number of molecules obtained is 2 to the power "n".

gladiator answered 1 year ago

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You want to use PCR to amplify a 1Kb exon of the human autosomal gene phenylalanine...

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