You think you have a culture of Streptococcus pyogenes, but you may have mixed it up...

You think you have a culture of Streptococcus pyogenes, but you may have mixed it up with another Gram-positive organism. By referring to the flow chart in this exercise, list in order the two tests you should do to confirm that the culture is in factStreptococcus pyogenes, AND explain what the correct result should look like for both tests.

Expert Solution

1. Bacitracin test.

2. PYR test.

Bacitracin sensitivity test is a specific test for confirmation of beta hemolytic Group A streptococci. A disc impreggnated with 0.04 IU is transferred to inoculated plates a zone of inhibition around colonies indicate presence of streptococcus pyogenes.

PYR test is used for confirmation of streptococcus pyogenes . The enzyme is seen in s.pyogenes. Pyrollidonyl arylamidase PYR is the enzyme which hydrolyse L-pyrolidonyl naphthylamide substrate to beta naphthylamine.The presence of this can be determined by N,N-methylaminocinnamaldehyde reagent which is indicated by ared precipitate. Small filter paper disc is impregnated with substrate after rubbing with inoculum which within two minutes gives red colour.

Enterococci of GroupD also gives a positive result but they are not beta hemolytic. Hence these test can be used as confirmatory test.

gladiator answered 2 years ago

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