In: Biology
Complementary DNA or cDNA is DNA produced on an RNA template by the action of reverse transcriptase (RNA-dependent DNA-polymerase). Then sequence of the cDNA becomes complementary to the RNA sequence.
bacterial cells are used to synthesize copies of eukaryptic proteins. .but there is one problem in eukaryotic gene or dna ..
in eukaryotic dna there is introns present ,which prokaryotic cell cannot remove ..so if we provide prokaryotic cell such Dna , it won't be able to form proteins copy. .
to overcome this problem...eularyotic mRNA is provided( no introns present in it ) ..from this cdna complementary are formed.then rnase H digest few part of rna.nd from these cdna complementary dna formed...now this ds dna provided to bacterial cell which can now easily transcribe and translate...