Question

which one is acid fast bacteria how it looks color and shape after staining acid fast stain are used to differentiate acid fast organism such mycobactria

Answer 1

Ans: Acid-fast bacteria are gram-positive, but in addition to peptidoglycan, the outer membrane or envelope of the acid-fast cell wall of contains large amounts of glycolipids, especially mycolic acids that in the genus Mycobacterium, make up approximately 60% of the acid-fast cell wall.

Ziehl-Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen staining is a bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria.

The primary stain used in acid-fast staining, carbolfuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat. The smear is then rinsed with a very strong decolorizer, which strips the stain from all non-acid-fast cells but does not permeate the cell wall of acid-fast organisms. The decolorized non-acid-fast cells then take up the counterstain which is Malachite green. So the acid fast bacteria will gives red color and non acid fast bacteria gives blue color after the completion of acid fast staining.

As no image is enclosed regarding the bacteria morphology, so i can only tell that the acid fast bacteria is the one stained in red color and morphology can be spherical, rod shaped or comma shape.