Pre-Lab Questions: What color will endospore negative bacteria be after endospore staining? What color will mycobacteria...

Pre-Lab Questions:

What color will endospore negative bacteria be after endospore staining? What color will mycobacteria bacteria after mycolic acid staining? (see the endospore and acid fast tables)

What species of bacteria are we going to use to look at endospores and what species will we use for mycolic acid staining?

Why do we briefly heat our bacteria on the slide before staining?

Acid-fast endospore staining

Expert Solution

-What color will endospore negative bacteria be after endospore staining?

Ans-Endospores will appear green in color while the vegetative cells will pink/reddish in color under the microscope.

-What color will mycobacteria bacteria after mycolic acid staining? (see the endospore and acid fast tables)

Ans-When stained with a Ziehl-Neelsen stain, the mycolic acids take on the color quickly and retain them inside the bacillus. Under the microscope, the bacillus is seen as a bright red rod, while the surface that it grows on is colored blue

-what species of bacteria are we going to use to look at endospores and what species will we use for mycolic acid staining?

Ans -Bacillus subtilis species of bacteria are we going to use to look at endospores

Mycobacteria spp. Mycobacteria spp use for mycolic acid staining.

-Why do we briefly heat our bacteria on the slide before staining?

Ans-The heat melts the waxy cell wall and permits the absorption of the dye by the cells. Then the slide is allowed to cool and a solution of acid and alcohol is added as a decolorizer. Cells that are “acid-fast” because of the mycolic acid in their cell wall resist decolorization and retain the primary stain.

gladiator answered 2 years ago

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