Assuming you are assigned a new project by your supervisor in a big Biotechnology company, CloneTech,...

Assuming you are assigned a new project by your supervisor in a big Biotechnology company, CloneTech, to produce recombinant human growth hormone please outline your plan step by step in order to accomplish the task. List some of the major equipment you must use for the project.

Expert Solution

Steps for production of HGF in large scale are

1) restriction digestion of both vector and genome

From genome we will get HGF gene,

2) amplification of HGF using PCR, and amplification of desired product will be observed using gel electrophoresis

3) ligation of digested vector and HGF gene form recombinant DNA.

4) transformation, it is be done using heat shock method

5) selection of E coli containing desired recombinant plasmid

6) laegr scale production using large culture vessels.

7) purification of HGF protein and its functional characterization using various enzymatic methods.

The whole process is explained in attached image below. Hope this will help.

gladiator answered 2 years ago

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