Question

Given the half of the DNA strand: 5'-GTTAGCCGTCTAATATGGTGAGATGTTATAACCCGCAGTTTACAACTTCAA-3' 1.At what part is of the peptide is where Arg located? At what part of the peptide is where the stop codon located? Where is the 5th cytosine located at the RNA strand? Where is the 6th uracil located at the RNA strand? How many Leu amino acids are transcribed? How many Pro amino acids are translated? What is the 7th amino acid translated? What is the 7th amino acid translated?

Answer 1

Ans) The sequence of amino acid information of a particular protein is present in DNA. The segment of DNA which actively codes for a protein is called a gene. The genetic information present in DNA is copied into mRNA by RNA polymerase then the information located in mRNA translated as a protein by ribosomes. The synthesis of RNA by using DNA as a template is called Transcription. The strand of the DNA whose sequence is complementary to mRNA it has given name template DNA strand or negative strand or nonsense strand. RNA polymerase bind to the promoter region located upstream of a gene and initiate the mRNA synthesis in denovo fashion. During transcription 5′ end of mRNA is released into cytoplasm from transcription bubble (DNA-RNA hybrid)

5′-GTTAGCCGTCTAATATGGTGAGATGTTATAACCCGCAGTTTACAACTTCAA-3′

3′- TTGAAGTTGTAAACTGCGGGTTATAACATCTCACCATATTAGACGGCTAAC-5′ comp DNA

5′-UUGAAGUUGUAAACUGCGGGUUAUAACAUCUCACCAUAUUAGACGGCUAAC-3′   mRNA

LKL.TAGYNISPY.TAN (Protein)

1) At what part is of the peptide is where Arg located?

Ans) the given DNA will not code for Arginine amino acid

2) At what part of the peptide is where the stop codon located?

Ans) fourth and fourteenth codons of mRNA are stop codons (UAA UGA)

5′-UUG AAG UUG UAA ACU GCG GGU UAU AAC AUC UCA CCA UAU UAG ACG GCU AAC-3′

3) Where is the 5th cytosine located on the RNA strand?

Ans) In the 11^th codon (31^st position) of mRNA 5th cytosine is located

5′-UUG AAG UUG UAA ACU GCG GGU UAU AAC AUC UCA CCA UAU UAG ACG GCU AAC-3′

4) Where is the 6th Uracil located on the RNA strand?

5′-UUG AAG UUG UAA ACU GCG GGU UAU AAC AUC UCA CCA UAU UAG ACG GCU AAC-3′

Ans) In the 5^th codon (15^th position) of mRNA 6th uracil is located

5) How many Leu amino acids are transcribed? (LKL.TAGYNISPY.TAN)

Ans) two Leucine amino acids

6) How are many Pro amino acids translated?

Ans) only one Proline amino acid (LKL.TAGYNISPY.TAN)

7) What is the 7th amino acid translated?

Ans) Tyrosine (LKL.TAGYNISPY.TAN)