In DNA extraction experiment of strawberries. What else might be in the ethanol/aqueous interface? How can...

In DNA extraction experiment of strawberries. What else might be in the ethanol/aqueous interface? How can this be eliminated?

Expert Solution

Organic solvents such as ethanol, chloroform, and isopropanol are used to precipitate nucleic acid. After cell lysis, phenol: chloroform treatment followed centrifugation precipitates proteins and cellular debris.

Ethanol is added to the supernatant to precipitate nucleic acid. The supernatant can still contain small RNAs and soluble salts that get localized to the ethanol/aqueous interface.

To remove these small molecules, we have to precipitate DNA for the second time so that DNA gets into the pellet and soluble salts and small RNAs are removed in the supernatant.

You can precipitate DNA with chilled ethanol and sodium acetate or Isopropanol.

gladiator answered 2 years ago

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