Can you tell me about DNA extraction. Everything you know, what is the purpose and applications...

Can you tell me about DNA extraction. Everything you know, what is the purpose and applications of it, what are the advantages and disadvantages. And please write the function of each chemical:

RBC lysis buffer
PBS
Trizol

Thank you in advance! I am forever grateful for your help.

Expert Solution

DNA extraction process

purpose and advantages

For studying the genetic causes of disease, for development of diagnostic and drugs, essential for carrying out forensic science, sequencing genomes , detecting bacteria and viruses etc

Disadvantage

it depends on source of dna , sonication can create DNA strand breaks

Function of PBS buffer is to maintain constant PH used in biological research

Trizol is used for RNA isolation from cells and tissues, it works by maintaining RNA integrity during tissue homogenisation, and breaking cells and cell components

RBC lysis buffer is used to break down RBC and this is ideal for isolation of DNA and RNA from bllod

gladiator answered 3 months ago

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