Question

In the DNA extraction from cheek cells, protocol you used cold ethanol to:

A.) Precipitate the DNA B.) Break open the nuclear membrane C.) Separate the nucleus from the other organelles D.) Denature the nuclear proteins

Answer 1

DNA isolation occurs in many steps

Coming to question

In DNA extraction of cheek cells cold ethanol is used as it dehydrate DNA and it become insoluble in water and precipitate and appear as white thread like structure in the tube and can be extracted

Use of chilled ethanol also yield more DNA as it prevent activation of DNAse enzyme which can split DNA which can result in low quantity of DNA extracted

Hence option A precipitate DNA is correct answer other option can be eliminated

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