Question

Ch 13: Eukaryotic Transcription Worksheet

This type of RNA Polymerase is responsible for synthesis of tRNA _________

This type of RNA Polymerase is responsible for synthesis of mRNA _________

This type of RNA Polymerase is responsible for synthesis of rRNA _________

This “element” decreases the rate of transcription _____________

This “element” increases the rate of transcription _____________

The locations of these elements maybe found _______________________________.

This protein is required for RNAP II, it cannot bind without it ____________

These protein molecules influence the rate of RNAP II initiation of transcription __________ will bind to enhancer sequences to aid RNAP II binding or ________ may bind to silencer sequences.

____________ was responsible for characterizing RNAP II in this organism

Eukaryotic RNAP II has a _________ (what charge) jaw-like clamp to hold the DNA.

Abortive transcription refers to the fact that the beginning of transcription in eukaryotes involves ________and ________ transcription because unstably bound ribonucleotides are added.

Once a stable RNA:DNA hybrid forms that is approximately ___ ribonucleotides long RNAP II will move along the DNA

RNAP II will hold ____ basepairs of DNA within the complex and ____ ribonucleotides.

The lid is the location where what happens?

The Pore is where _____________ will enter the complex to be used during transcription.

When the ________sequence arrives in the complex, the complex becomes unstable. The clamp opens and both the _____ and nascent ______ strand are released.

This precursor to the mature and fully processed mRNA is called _______________ ________RNA or a primary transcript.

Other types of post transcriptional modifications include addition of this modified nucleotide to the 5’ end, it is called a __________________

What is unique about its attachment to the adjacent ribonucleotide?

What are some reasons for its addition?

How long is a typical 3’ poly A tail? Do all eukaryotic mRNAs have a poly A tail?

(True or False) Introns do not make up a significant portion of the length of the genomic gene sequence. (In other words, the length of the intronic DNA is much shorter than the exonic DNA)

Group 1 splicing mechanism involve catalytic _____ molecules called _________; these are non protein molecules with enzymatic activity.

Group 1 splicing mechanisms involve removing introns in primary transcripts of _____

Introns in mRNAs are removed using a large complex structure called a ___________ with the help of binding of small nuclear RNAs bound to proteins called ____________

Small nuclear RNAs have a high concentration of this ribonucleotide_________

A self splicing mechanism involving the formation of a ________ structure following snRNA’s complementary binding will cut the RNA and allow exons to be ligated.

Answer 1