Question

In: Biology

describe the uses of northern, southern and western blot in animal science and the veterinary field.

describe the uses of northern, southern and western blot in animal science and the veterinary field.

Solutions

Expert Solution

Western blotting is used to detect nanograms of protein molecules in a mixture of other protein . It detect different chemical reaction going in the cell . It identify specific protein . It detect total proteome inside the cell and also protein protein interaction for physiological and cellular processes .

Southern blotting identify target DNA with DNA hybridisation technique . It identifies specific DNA . It also helps in identification of viral infection and in study of mutation and gene arrangement . It is used in phylogenetic studies , payernity and maternity analysis . Or simply speaking it is used in DNA fingerprinting . It identifies mutation and deletion . It is also used to detect lyme disease . It is used to detect auto antibodies that causes auto immune disease .

Northern blotting - used to detect specific RNA from mixture of RNA . It is used in mRNA studies and gene expression analysis .


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