In: Biology
Western blotting is used to detect nanograms of protein molecules in a mixture of other protein . It detect different chemical reaction going in the cell . It identify specific protein . It detect total proteome inside the cell and also protein protein interaction for physiological and cellular processes .
Southern blotting identify target DNA with DNA hybridisation technique . It identifies specific DNA . It also helps in identification of viral infection and in study of mutation and gene arrangement . It is used in phylogenetic studies , payernity and maternity analysis . Or simply speaking it is used in DNA fingerprinting . It identifies mutation and deletion . It is also used to detect lyme disease . It is used to detect auto antibodies that causes auto immune disease .
Northern blotting - used to detect specific RNA from mixture of RNA . It is used in mRNA studies and gene expression analysis .