In a comparative chart describe how Southern, Northern & Western Blotting technologies enable animal biotechnology.

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Answer :

Comparative Chart.

	SOUTHERN BLOT	NORTHERN BLOT	WESTERN BLOT
Target molecule	DNA	RNA	PROTEIN
Sample separation	DNA extraction, enzymatic digestion.	RNA isolation	Protein extraction
Separation	Electrophoresis	Electrophoresis	Electrophoresis
Membrane material	Nylon	Nylon	Nitrocellulose
Probe	Nucleicacid probe with sequence homologous to target	RNA, DNA or oligodeoxy nucleotide	Primary antibody
Probe label	Radio label, enzyme	Radio label, enzyme	enzyme
Detection methods	X- ray film, chemiluminescence	X ray film, chemiluminescence	Film, camera, cooled ccd, LED or infrared imaging system.

Fragile X syndrome and sickle cell anemia is very well understood by Southern blotting.
Overexpression of oncogenes and downregulation of tumour suppressor gene is well studied by northern blot in biotechnology.
Confirmatory HIV test employs Western blotting in biotechnology.

gladiator answered 2 years ago

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