Question

1. Do you think all cell lines will respond the same way to the growth factors? Construct a hypothesis for this experiment.

EXPERIMENT IS: Transfer each cell line into three new flasks for a total of nine flasks. For each cell line: one flask gets platelet-derived growth factor (PDGF); one gets epidermal growth factor (EGF); and one gets no growth factors. Allow cells to grow for 24 hours. View cells under the microscope, and count cells in three fields of view.

Answer 1

Please not that, not all growth factors are act in same manner because it also depends on the cell type and the proliferation rate of the specific cell lines under the influence of specific growth factor(s).

In the given scenario the experiment is divided into three groups and all groups are in triplicate 3x3=9 flasks

Group 1: Cells with PDGF (in triplicates)

Group 2: Cells treated with EGF (in triplicates)

Group 3: Cells with no growth factor (neither PDGF or EDF) (in triplicates)

After incubating then for 24 h at optimum temperature, CO2 and humidity, analyze the flasks under microscope and select various zones of flask to get a better idea of the cells

Cells with no growth factor will show minimum proliferation that can be examined on the basis of the confluency of the cells. Flask of group 1 and group 2 will exhibit better proliferation as compared to the group three. The proliferation of cells in the group 1 and group 2 (growth of the cells are dependent of the proliferation rate, doubling time of the cells) depend on the effectiveness of the growth factors and dose of these factors (although both are essential for the metabolic process of the cells subsequently their proliferation).