Dideoxynucleotides terminate translation transformation DNA replication Transcription Crisper editing

Dideoxynucleotides terminate

translation
transformation
DNA replication
Transcription
Crisper editing

Expert Solution

Ans) The correct answer is "C" i.e DNA Replication

Dideoxynucleotides are commonly used in sanger sequencing or chain termination sequencing.Dideoxynucleotides lacks 3' OH. We know that DNA replicaion occurs in 5' --> 3' direction by adding nucleotides to the 3' OH and this is how the primer will be extended.But the incorporation of dideoxynucleotides (ddNTPs) will lack the further addition of nucleotides to the 3' OH and the DNA Replication will be prevented.

Option A is incorrect because in translation process dideoxynucleotides are not used.In translation,the ribosome decode the mRNA sequence and the appropriately charged tRNAs will facilitate the process of translation.

Option B is incorrect because in transformation process,the use of competent cells to take up the foreign DNA from the external environment will occur.

Option D is incorrect because in the Transcription, the ribonucleotides are added to the 3' OH of the growing polypeptide chain.So,the dideoxynucleotides are not used.

Option E is incorrect.CRISPR editing does not require dideoxynucleotides.

gladiator answered 1 year ago

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