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Describe the role of complementary base paring in DNA replication, transcription, translation and hibridization probe technique.

Describe the role of complementary base paring in DNA replication, transcription, translation and hibridization probe technique.

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Expert Solution

COMPLEMENTARY BASE PAIRING:-
(A) DEFINATION:- the nucleotide bases linked by a hydrogen bond on opposite strands of DNA or double-stranded RNA.
-guanine is the complementary base of cytosine.
- adenine is the complementary base of thymine in DNA and of uracil in RNA.

(B) IMPORTANCE IN REPLICATION, TRANSCRIPTION, TRANSLATION AND DNA HYBRIDISATION TECHNIQUE:-
- Complementary base pairing is very important in the conservation of the base sequence of DNA. -This is because adenine always pairs up with thymine and guanine always pairs up with cytosine.
- In DNA replication this complementary base pairing allows the two DNA molecules to be identical to each other as they have the same base sequence.
- The new strands formed are complementary to their template strands but also identical to the other template. Therefore, complementary base pairing has a big role in the conservation of the base sequence of DNA.


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