In: Biology
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ANSWER :-
Answer of questions 1 and 4 .
2. Add one drop of methylene blue to the water and cell solution to stain the cheek cells. This will allow you to more easily see them during observation.
3.A stage micrometer is referring to a slide that comes with a scale on its surface. The sides are mounted with a reticle scale that is used for calibrating the reticles of the eyepiece as well as the objective powers. Although most stage micrometers are made of glass, they are also composed of metal especially for dissecting microscopes.
5.Follw these steps for the focusing of an object :-
*start by rotating the objective lens to lowest power.
*Place a slide on the stage, label side up, with the coverslip centered.
*On low power only use the coarse focus knob to get the object into focus.
*If you cannot see anything, move the slide slightly while viewing and focusing.
*If nothing appears, reduce the light and repeat step 4.
*Once in focus on low power, center the object of interest by moving the slide.
*Rotate the objective to the medium power and adjust the fine focus only.
*If needed, rotate the objective to the high power and adjust fine focus only.