In: Biology
The polymerase that synthesizes which of the following molecules uses RNA as a template and synthesizes new stands from 5' to 3'?
1. Neither RNA polymerase nor DNA polymerase
2. both RNA polymerase and DNA polymerase
3. DNA polymerase only
4. RNA polymerase only
1) Neither RNA polymerase nor DNA polymerase.
Both DNA polymerase and RNA polymerase use DNA as a template for initiating the process.
DNA polymerase is one of the important enzyme which is involved in DNA replication process.
Three different types of DNA polymerase is there,each one does different role in replication process.
Generally DNA polymerase are called as template directed enzymes.It has 5'-3' exonuclease activity ,3'-5' exonuclease activity.So for producing many copies of DNA ,it takes DNA strand as template with the help of helicase unwinding of duplex occurs and Primase adds primers to each strand ,DNA polymerase adds nucleotides to the 3'end of the primers and extends the strand and produce complementary strand with the template.
RNA polymerase is a main enzyme in transcription process for synthesizing RNA.Single strand of DNA is used as template and RNAs are synthesised.
Reverse transcriptase is the enzyme utilise RNA as template and produce cDNA first strand in 3'-5' direction.RNA replicase use RNA as template and involved in replication of RNA seen in viruses with RNA as genome and synthesis product in 3'-5' direction.
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