A chromatin immunoprecipitation (ChIP) experiment for RNA polymerase II (RNAP2) is carried out, specifically to tell...

A chromatin immunoprecipitation (ChIP) experiment for RNA polymerase II (RNAP2) is carried out, specifically to tell the difference between RNAP2 bound to the promoter and RNAP2 that has escaped the promoter but has not yet begun to fully elongate (RNAP2 serine 5 phosphorylated).

Describe two issues that might come up when designing your experiment to obtain the type of data you desire. Also provide a solution to address these two issues.

Expert Solution

gladiator answered 2 years ago

You want to perform chromatin immunoprecipitation (ChIP) experiments for RNA polymerase II (RNAP2), specifically to tell...

You want to perform chromatin immunoprecipitation (ChIP) experiments for RNA polymerase II (RNAP2), specifically to tell the difference between RNAP2 bound to the promoter and RNAP2 that has escaped the promoter but has not yet begun to fully elongate (RNAP2 serine 5 phosphorylated). Describe two issues you might have to deal with when designing your experiment to obtain the type of data you desire. also provide a way you might fix these problems

In order to make a Chromatin Immunoprecipitation (ChIP) dilution buffer, you need to make some concentrated...

In order to make a Chromatin Immunoprecipitation (ChIP) dilution buffer, you need to make some concentrated stock solutions first. Describe how to make the following solution: make 500 mL of a 1 M Tris-Cl pH 8.0 stock solution (Tris base is a solid with a FW: 121.14 g/mol)  

Discuss the structure of promoter for RNA Polymerase II

Please summarize the function of RNA polymerase II carboxy-terminal domain (CTD) in RNA processing for proper...

Please summarize the function of RNA polymerase II carboxy-terminal domain (CTD) in RNA processing for proper mRNA function

Compare the proofreading mechanisms of DNA and RNA polymerase. Specifically, which active site is required for...

Compare the proofreading mechanisms of DNA and RNA polymerase. Specifically, which active site is required for proofreading by each enzyme and what are the products of this activity?

One way to discover promoters is through an experiment called RNA polymerase protection assay, in which...

One way to discover promoters is through an experiment called RNA polymerase protection assay, in which RNA polymerase binds to a gene, is cross-linked to the DNA, and then the DNA is subjected to fragmentation, which breaks apart any DNA that is not protected by cross-linked protein. Explain why this would help to identify promoters in genes.

"RNA polymerase II activity depends on both cis-regulatory elements and trans-acting factor A) What is the...

"RNA polymerase II activity depends on both cis-regulatory elements and trans-acting factor A) What is the main difference between cis-regulatory elements and trans-acting factors? (Hint: which of the two are DNA sequences, and which one are proteins?) B) Name and briefly describe the three cis-regulatory elements we have studied in eukaryotes" University-level genetics course, please keep answer as simple and to the point as possible (preferably in bulleted list)... Thank you!

Describe the cis-acting elements that act as core promoter elements for RNA polymerase II transcription. Indicate...

Describe the cis-acting elements that act as core promoter elements for RNA polymerase II transcription. Indicate the role of each element and the trans-acting factor that interacts with the element.

An experiment was carried out to investigate the effect of species (factor A, with I =...

An experiment was carried out to investigate the effect of species (factor A, with I = 4) and grade (factor B, with J = 3) on breaking strength of wood specimens. One observation was made for each species—grade combination—resulting in SSA = 443.0, SSB = 424.6, and SSE = 125.4. Assume that an additive model is appropriate. (a) Test H0: α1 = α2 = α3 = α4 = 0 (no differences in true average strength due to species) versus Ha:...

An experiment was carried out to study the effect of the percentage of ammonium (Factor A)...

An experiment was carried out to study the effect of the percentage of ammonium (Factor A) and the stir rate (Factor B) on the density of the powder produced. The results are in the accompanying table. Complete parts (a) through (e). At the 0.01 level of significance is there an interaction between the percentage of ammonium and the stir rate? - Stir_Rate - Percent_of_Ammonium 100 150 2% 10.83 8.49 2% 14.64 6.84 2% 18.46 9.05 2% 15.86 9.54 30% 12.29 ...

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