A chromatin immunoprecipitation (ChIP) experiment for RNA polymerase II (RNAP2) is carried out, specifically to tell...

A chromatin immunoprecipitation (ChIP) experiment for RNA polymerase II (RNAP2) is carried out, specifically to tell the difference between RNAP2 bound to the promoter and RNAP2 that has escaped the promoter but has not yet begun to fully elongate (RNAP2 serine 5 phosphorylated).

Describe two issues that might come up when designing your experiment to obtain the type of data you desire. Also provide a solution to address these two issues.

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gladiator answered 1 year ago

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You want to perform chromatin immunoprecipitation (ChIP) experiments for RNA polymerase II (RNAP2), specifically to tell...

You want to perform chromatin immunoprecipitation (ChIP) experiments for RNA polymerase II (RNAP2), specifically to tell the difference between RNAP2 bound to the promoter and RNAP2 that has escaped the promoter but has not yet begun to fully elongate (RNAP2 serine 5 phosphorylated). Describe two issues you might have to deal with when designing your experiment to obtain the type of data you desire. also provide a way you might fix these problems

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