Which of the following is NOT true of the HTH motif of
DNA-binding proteins?
Helix 2 stabilizes the structural domain with Helix 3
through hydrophobic interactions.
Helix 3 situated near the N-terminal of the protein is
the DNA recognition helix.
Helix 2 is the helix at the beginning of the HTH
motif.
There are 2 successive alpha helices separated by a
sharp beta-turn.
In regards to protein function:
A) For specific DNA binding proteins,
1. what is the structure of a typically DNA binding protein and
how does it interact with DNA?
2. why is there a difference between the major and amino groove
in terms of protein binding?
B) describe how the Lac repressor interacts with DNA to find its
binding site
C) describe how a catalyst lowers the activation energy of a
reaction
D) how is the structure of enzymes used...
5. Briefly define the following option strategies (2 marks each
=8 marks)
5.1. Protective put
5.2. Covered call
5.3. Straddle
5.4. Spread
6. The manager of a large portfolio includes $100 million worth
of long-term bonds paying an average coupon rate of 7%. The manager
believes that interest rates are about to rise. Explain n how he
can address his concerns using interest rates swaps. In your
answer, include a clear definition of an interest rate swap (5)
Cro and cI proteins both have helix-turn-helix DNA binding
domains. What effect would replacing cro’s DNA binding domain with
that of cI’s have on the life-cycle of lambda phage? Explain
briefly
Transcriptional initiation at defined sequences in DNA is
required to ensure that the 5' end of the RNA transcript is
correct. Describe the two types of experimental approaches
that have been used to identify regulatory DNA sequences located
near gene start sites.
Week 11 Tutorial 6 (a) Identify and briefly describe 5 Global
Ethical issues. (5 marks – 200 marks) (b) Using a country of your
choice, discuss the Global Ethical issues you have listed in 6 (a).
(6 marks – 200 words).
Both polypeptides (proteins) and polynucleotides (RNA
and DNA) are characterized by their primary structure. Briefly
define that term with reference to each type of biomolecule. Then,
briefly describe the connection between the two - that is, how the
primary structure of nucleic acids translates to the primary
structure of a protein.
Describe the process of protein synthesis starting with DNA in
the nucleus and ending with proteins released from the ribosomes.
Include the role of the DNA, mRNA, tRNA, rRNA and amino acids.