Cultured liver hepatocytes are convenient for studying both
exocytosis and receptor-mediated, clathrin-dependent endocytosis.
As part of an advanced laboratory in cell biology, students are
required to perform mutation studies on cultured liver hepatocytes
to generate data that supports the proposed mechanism of
receptor-mediated, clathrin-dependent endocytosis. Liver
hepatocytes are cultured, mutagenized, and screened for defective
receptor-mediated endocytosis. In order to distinguish unique
mutants from one another, the students must design a series of
experiments to distinguish between mutants that have the...