Cultured liver hepatocytes are convenient for studying both exocytosis and receptor-mediated, clathrin-dependent endocytosis. As part of...

Cultured liver hepatocytes are convenient for studying both exocytosis and receptor-mediated, clathrin-dependent endocytosis. As part of an advanced laboratory in cell biology, students are required to perform mutation studies on cultured liver hepatocytes to generate data that supports the proposed mechanism of receptor-mediated, clathrin-dependent endocytosis. Liver hepatocytes are cultured, mutagenized, and screened for defective receptor-mediated endocytosis. In order to distinguish unique mutants from one another, the students must design a series of experiments to distinguish between mutants that have the following mutations:

a). Cellular adaptor proteins are mutated to be non-functional

b). Clathrin is mutated to be non-functional

c). Dynamin is mutated to be non-functional

Expert Solution

Endocytosis: It is the process of passing the molecule from outer cellular environment to the intra cellular space across the membrane via receptors present on the membrane.

In this activated molecule comes near to the membrane where they were recognised by the compatible receptor. Then the membrane fold to form the pit lined by the clathrin molecule. This pit further convert into a vesicle. It was detached from the membrane with the help of clathrin molecule in association with interacellular Dynamin molecule.

a) In the first experiment cellular adaptor molecule become non-functional unable to recognise the incoming molecule this will not activate the downstream process of endocytosis.

b) In the second experiment incoming molecule is recognised and membrane fold to form pit but due to unavailable the clathrin molecule protect it to formed the vesicles.

C) In the third experiment molecule is recognised and vesicles are formed but they are unable to detach from the plasma membrane for gone downstream processes.

gladiator answered 1 year ago

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