I will simply execute an extraction of caffeine experiment.
This is a typicall
approach:
- The drink must be at room
T.
- You will need to use
dichloromethane which will help us in the extraction
- Cool the drink since the
addition of dichloromethane will increase in
temperature
- Mix and shake
container
- Vent tubes for fumes
- Centrifuge the
sample
- Pour 10 mL to an Erlenmeyer
flask.
- Time to add a fresh 3 mL of
dichloromethane
- Now you should have 2 organic
layers
- Mix/Swirl it and look at the solid.
- Add small amounts of sodium
sulfate until some of the grains
- Let the flask rest (5mins
minimum)
- Decant the dichloromethane
solution
- Take sample for Thin Layer
Chromatography
- Allow the dichloromethane to
evaporate in the hood while you are performing the TLC
experiment.
- What remains on the watch glass
after the solvent evaporates should be caffeine.
- Weight and make the calculations
needed
NOTE that if the drink is
caffeine free, you should not see any materiial in step
16