Thin Layer Chromatography 1. After running the first plate, the spot was not observed. Provide two...

Thin Layer Chromatography

1. After running the first plate, the spot was not observed. Provide two possible reasons (not including operator error) as to why?

2. What other technique could be used to determine if a reaction was complete? Explain to a peer, how you would do this in lab.

3. Why might TLC not be the best technique to determine the identity of a compound?

Expert Solution

queen_honey_blossom answered 2 years ago

Thin Layer Chromatography utilizes a glass or metal plate coated with a thin layer of absorbent...

Thin Layer Chromatography utilizes a glass or metal plate coated with a thin layer of absorbent material such as silica gel. The sample is prepared similiarto paper chromatography and a solvent is used to elute the sample. 1. Which is the stationary phase? Plate Silica Gel Solvent 2. Which is the mobile phase? Plate Silica Gel Solvent 3. Which component is inert? Plate Silica Gel Solvent

Give two advantages of thin layer chromatography over paper chromatography.

A chemist runs a thin-layer chromatography plate and marks the solvent as having traveled 6.1 cm...

A chemist runs a thin-layer chromatography plate and marks the solvent as having traveled 6.1 cm up the plate. After visualization, the distance of the spot of interest is measured as having traveled 2.7 cm. Select the correct Rf value of this spot

Thin layer chromatography in the organic chemistry typically uses a glass plate coated with silica gel,...

Thin layer chromatography in the organic chemistry typically uses a glass plate coated with silica gel, which serves as the stationary phase. The mobile phase in the experiment is an organic solvent or mixture of organic solvents. The mobile phase pulls the organic material(s) being analyzed up the plate by dissolving them in the solution. So, the more something dissolves in the mobile phase the further up the plate it moves. The mobile phase in this experiment will involve which...

Explain and compare thin layer chromatography with column chromatography (in-detailed answer)

When would the application of thin-layer chromatography, adsorption column chromatography, and gas chromatography be appropriate to...

When would the application of thin-layer chromatography, adsorption column chromatography, and gas chromatography be appropriate to use?

Compare and contrast Column (solid-liquid) chromatography, Paper Chromatography, and thin layer chromatography in respect to the...

Compare and contrast Column (solid-liquid) chromatography, Paper Chromatography, and thin layer chromatography in respect to the size of the sample that can be separated, the ease of use, and the versatility of the method.

Compare the advantages and limitations of column chromatography with those of thin-layer and gas chromatography. Discuss...

Compare the advantages and limitations of column chromatography with those of thin-layer and gas chromatography. Discuss all 3 chromatography methods. When you would use them and in which case is each useful and not useful?

Summarize the procedure of Thin Layer Chromatography (3-4 sentences)

Questions regarding Thin Layer Chromatography Analysis (TLC): 1. What is the purpose of the wick? 2....

Questions regarding Thin Layer Chromatography Analysis (TLC): 1. What is the purpose of the wick? 2. Why is it important to use diluted solutions? 3. Why is it important to spot the TLC plate quickly and lightly? 4.What solvent will use to develop your TLC plates? ethyl acetate, hexanes, or some mixture of the two? Why? 5. List at least 3 common errors when performing TLC and how to fix them.

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