Describe 3 ways to determine whether a protein is a transmembrane or peripheral protein.

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Expert Solution

In transmembrane protein Hydrobhobic aminoacids are more as compared to hydrophilic amino acid and reverse is true for peripheral protein
Peripheral protein is attached to plasma membrane from one end so only that end contain hydrophobic amino acids other amino acids are present in extra cellular fluid so those amino acid must be hydrophilic and In case of transmembrane protein almost complete protein embedded in the plasma membrane so almost all amino acids are hydrophobic amino acid to interact with the phospholipid bilayer.
Peripheral proteins are loosely attached to the plasma membrane so peripheral proteins dissociate following treatment with a polar reagent or high salt concentrations and trans-membrane proteins embedded inside the membrane so can not seperated easily, transmembrane proteins can be seprated from the biological membrane only with detergents, nonpolar solvents , denaturing agents.

gladiator answered 1 year ago

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