Electrophoresis & Sedimentation:
Compare electrophoresis and sedimentation as methods for
separation of macromolecular mixtures. In addition,...
Electrophoresis & Sedimentation:
Compare electrophoresis and sedimentation as methods for
separation of macromolecular mixtures. In addition, discuss the
methods in terms of quantitative analysis of three-dimensional
structure.
Set up a table and compare and contrast these
4-chromatographic/separation techniques (Gas Chromatography, Liquid
Chromatography, Electrophoresis, Size Exclusion Chromatography).
Make sure to include what characteristic the separation is based
on, the mobile phase, the stationary phases, and an available
detection method for each technique. Thank you!
Compare the methods of capillary electrophoresis and high
performance liquid chromatography. For each method, give an example
of when it would be more advantageous than the other one.
Compare the methods of capillary electrophoresis and
high performance liquid chromatography HPLC. For each method, give
an example of when it would be more advantageous than the other
one.
Electrophoresis & Sedimentation:
Explain why a teardrop shaped particle would not have a smaller
frictional coefficient than a sphere. Why would a teardrop or
elipsoid particle not possess superior streamlining compared to a
sphere?
For each of the following mixtures of compounds, choose the best
combination of separation method and detector that would be able to
separate and analyze the mixture. a. a mixture of C7-C10 alkyl
halides.(bp 150-200o C) Reverse Phase LC-MS CE-Fluorescence Normal
phase LC-Absorbance GC-MS GC-ECD b. a mixture of amino acids in pH
2 buffer solution Reverse Phase LC-MS GC-ECD GC-MS Normal phase
LC-Absorbance CE-Fluorescence c. a round bottom flask containing an
organic starting material, product and possible byproducts. Assume...
Electrophoresis is a general term that describes the migration
and separation of charged particles (ions) under the influence of
an electric field. An electrophoretic system consists of two
electrodes of opposite charge (anode, cathode), connected by a
conducting medium called an electrolyte. The separation effect on
the ionic particles results from differences in their velocity
(v), which is the product of the particle's mobility
(m) and the field strength (E):
v = mE
The mobility (m) of an ionic
particle...
Compare and contrast native gel electrophoresis and denaturing gel
electrophoresis. Which method would be used to determine the
molecular weight of a protein, why?