Question

Suggest applications of the following biotechnologies:
a. recombinant DNA of a single cell
b. a transgenic organism
c. gene silencing
d. gene expression profiling
e. genome editing

Answer 1

a. Application of recombinant DNA of a single cell

1. Therapeutic application: used in the production of human protein, Insulin, hGH, INF- alpha.
2. Vaccines: Cells and viruses can modify to pathogen surface proteins such as hepatatis B, influenza, cervical cancer vaccine, DNA vaccines etc.
3. Gene therapy by replacing defective gene.

b. Application of a transgenic organism

1. Improvinge plants by producing new varieties, high yield, and tolerance againts insect.
2. Improving live stock to produce organism with faster growth and efficient, more productive and resistant of disease.

c. Application of gene silencing

1. Gene silencing use RNA in cell culture
2. This technology used in the cancer treatments.
3. Application in andrology as well as urology.
4. RNA i Modulation of HIV-1

d. Application of gene expression profiling

1. Monitor mRNA for development and differentiation of disease stages.
2. Comparison of different organism’s genome can be done.
3. Alternative splicing detection by using probe
4. Used to detect SNPs.

e. Application of genome editing

1. In medicine used for drug development and gene surgery.
2. In biology tool used as animal models and gene variation.
3. In biotechnology it used as food as well as biofuel industry.

Hope it will helpful to you. Thank you

All the best