Question
In: Chemistry
BioChem Question I am trying to make a phosphate buffer, but I only have monoprotic potassium...
BioChem Question
I am trying to make a phosphate buffer, but I only have monoprotic potassium phosphate. Should I expect the pH to be low or high once I dissolve the solid in water? Does this mean I should expect to need acid or base to adjust the pH to 7.0?
Would formic acid or acetic acid be a better buffer at pH 5.0? Why?
Which buffer would be my best choice if I need my reaction to be run at pH 7.6?
Solutions
Expert Solution
- A buffer (more precisely, pH buffer or hydrogen ion buffer) is an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa. Its pH changes very little when a small amount of strong acid or base is added to it and thus it is used to prevent changes in the pH of a solution.
When Monoprotic potassium phosphate (KH2PO4) was dissolved in the water, pH of that solution ranges from 6.2 to 8.2 and pKa = 7.2,so you have to add acid or base to adjust the pH to 7.0
- formic acid or acetic acid be a better buffer at pH 5.0? Why?
See the following table which gives the Weak Acids, Ka, and pKa values
Acetic acid pKa = 4.8 pH = 3.8 – 5.8
Formic acid pKa = 1.7 pH = 3.75
According to the pH of the acetic acid and formic acid solution ,these are the best options for buffer at pKa = 5.0
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