Question

In: Biology

1. What would be the power of magnification when using a microscope with a 10x ocular...

1. What would be the power of magnification when using a microscope with a 10x ocular lens and a 45x objective lens?

  • 10x

  • 45x

  • 55x

  • 450x

  • 35x
    2. A Petri dish containing isolated colonies of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa would be described as being

  • a pure culture.

  • a mixed culture.

  • a contaminated culture.

  • an axenic culture.
    3. What type of media would be the best choice for isolating a bacterial colony

  • nutrient broth

  • motility media

  • trypticase soy agar

  • trypticase soy broth
    4. Mannitol salt agar contains (among other ingredients) extracts of animal tissue and mixed proteins, along with sodium chloride, which inhibits the growth of organisms other than those in the genus Staphylococcus. It also includes mannitol, which is fermented by Staphylococcus aureus, turning the medium yellow. Other species of Staphylococcus (which do not ferment mannitol) will still grow, but the medium remains red in color. Mannitol salt agar could be classified as

    Check All That Apply

  • a solid medium.a solid medium.

  • a liquid medium.a liquid medium.

  • a differential medium.a differential medium.

  • a selective medium.a selective medium.

  • a complex medium.a complex medium.

  • a defined medium.

Solutions

Expert Solution

1 To calculate the total magnification multiply the power of ocular lens and power of objective lens ,thus here the power of ocular lens is 10X and power of objective lens is 45X ,so

10X * 45X = 450X

Thus answer is option D 450X

2 B Mixed culture

It is called mixed culture because it contains more than one type of organism growing in a sterile medium .It is not contaminated culture as it is not contaminated from any foreign bodies and also not showing any signs like turbidity and white cloudy appearance.

3 b Motility media

as it separates between non motile and motile bacterial colonies based on their ability to move away from the line of inoculation

4 Mannitol salt agar -

Solid medium,Differential medium,selective medium,defined media

MSA is a solid state media that is both selective and differential ,and is made up of biochemicals rather than materials of biological origin seen in compex media.


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