Question

3) In what circumstances would you use an upright, an inverted and a fluorence microscope?

4) Would you use the same filter cube for every fluorophore you use in your experiment?

SUBJECT IS ON USING MICROSCOPES

Answer 1

QUESTION 3.

Upright microscope is used when sample is observed from above while inverted microscope is used when sample is observed from below. The optics od inverted microscope is placed under the sample.

Traditionally, inverted microscopes are used for life science research, because gravity makes samples sink to the bottom of a holder with aqueous solution and you don’t see a lot from above. But for some time, inverted microscopy has become increasingly popular for industrial applications as well.

Fluorescence microscopy technique is used to study specimens, which can be made to fluoresce. The fluorescence microscope is based on the phenomenon that certain material emits energy detectable as visible light when irradiated with the light of a specific wavelength.

QUESTION 4.

Same filter cube can't be used for every fluorophore or fluorescent protein since a filter set is designed to capture the maximum excitation and emission wavelengths of a given fluorophore.

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