How would you describe the differences in appearance between a phase contrast image and a DIC...

How would you describe the differences in appearance between a phase contrast image and a DIC image of a cheek cell?

Expert Solution

Cheek cells are eukaryotic cells.The tissue that lines the inside of the mouth is known as the basal mucosa and is composed of squamous epithelial cells.so Phase contrast microscopes are mainly used to examine specimens that are the same color as their background and need a bit of "pop" in order to differentiate them. Samples where staining is not an option (or might kill the specimen) often utilize phase contrast.cheek cell staining in methylene blue are not clearly seen(optical properties, dynamics, and morphology)in bright field microscope.

fig 1.In the image above you may notice a few blue highlights around several of the cheek cells.

in DIC image Many microscopic specimens are colorless, nearly transparent, and relatively thick, such as tissue sections.Differential Interference Contrast (DIC) is optically a rather complicated method requiring several special optical components.in cheek cell under DIC image microscopy Numerically solving for the propagation of light in a series of through-focus DIC images allows linear phase information in a single slice to be completely determined and restored from DIC intensity values.cheek cells are frequently used in diagnostic tests, such as paternity determination, and DNA fingerprinting efforts and examining the sodium transport level of squamous epithelial cells in children to determine whether or not they have a predisposition for developing hypertension.

fig 2.DIC image of cheek cell.

gladiator answered 4 days ago

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