Question

Assessment sequence 2

MGKEEKTHINVVVIGHVDSGKSTTTGHLIYQCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERER

GITIDIALWKFETPKYYVTVIDAPGHRDFIKNMITGTSQADCAILIIAAGTGEFEAGISKDGQTREHALL

AYTLGVRQLIVAINKMDTAKWAEARYQEIIKETSNFIKKVGYNPKTVAFVPISGFHGDNMLTPSTNCPWY

KGWEKEGKSGKVTGKTLLDAIDAVEPPKRPTDKPLRLPLQDVYKIGGIGTVPVGRIETGVLKPGMVVTFA

PSNVTTEVKSVEMHHEQLVEGVPGDNVGFNVKNVSVKEIRRGNVAGDSKNDPPSGAATFNAQVIVLNHPG

QVGNGYAPVLDCHTAHIACKFTEILEKIDRRTGKSVENNPKFIKSGDAAIVKLTPSKPMCVEAFTDYPPL

GRFAVRDMRQTVAVGVIKSVEKATAGAGKVTKSAAKAAKK

A) For nucleotide sequence, what is the protein (coded for by the gene for a nucleic acid sequence), and in what biological process is it involved?

B) What is the stop codon

C) How many nucleotides in the coding sequence

D) How many amino acid translated

E) Is the organism a prokaryote or eukaryote

Answer 1

A)

• The genetic information in the fragment of DNA or a gene, may be expressed by its ability to encode a specific RNA or protein.
• The product thus generated typically “expresses” the genetic information of the gene. As described by the hypothesis of George Wells Beadle and Edward Lawrie Tatum, “One gene one enzyme”; the product of each genetic expression influences the activity of the cell.
• Thus, the process of transcription occurs to produce mRNA and translation to produce protein is defined as the central dogma.

DNA - > mRNA - > Protein

B)

Stop codon:

• Translation starts from the start codon sequence (AUG) and ends at Stop codons (UAG, UGA, UAA)
• UAG – Amber
• UAA- Ochre
• UGA- Opal

C)

atgggcaaagaagaaaaaacccatattaacgtggtggtgattggccatgtggatagcggc

aaaagcaccaccaccggccatctgatttatcagtgcggcggcattgataaacgcaccatt

gaaaaatttgaaaaagaagcggcggaactgggcaaaggcagctttaaatatgcgtgggtg

ctggataaactgaaagcggaacgcgaacgcggcattaccattgatattgcgctgtggaaa

tttgaaaccccgaaatattatgtgaccgtgattgatgcgccgggccatcgcgattttatt

aaaaacatgattaccggcaccagccaggcggattgcgcgattctgattattgcggcgggc

accggcgaatttgaagcgggcattagcaaagatggccagacccgcgaacatgcgctgctg

gcgtataccctgggcgtgcgccagctgattgtggcgattaacaaaatggataccgcgaaa

tgggcggaagcgcgctatcaggaaattattaaagaaaccagcaactttattaaaaaagtg

ggctataacccgaaaaccgtggcgtttgtgccgattagcggctttcatggcgataacatg

ctgaccccgagcaccaactgcccgtggtataaaggctgggaaaaagaaggcaaaagcggc

aaagtgaccggcaaaaccctgctggatgcgattgatgcggtggaaccgccgaaacgcccg

accgataaaccgctgcgcctgccgctgcaggatgtgtataaaattggcggcattggcacc

gtgccggtgggccgcattgaaaccggcgtgctgaaaccgggcatggtggtgacctttgcg

ccgagcaacgtgaccaccgaagtgaaaagcgtggaaatgcatcatgaacagctggtggaa

ggcgtgccgggcgataacgtgggctttaacgtgaaaaacgtgagcgtgaaagaaattcgc

cgcggcaacgtggcgggcgatagcaaaaacgatccgccgagcggcgcggcgacctttaac

gcgcaggtgattgtgctgaaccatccgggccaggtgggcaacggctatgcgccggtgctg

gattgccataccgcgcatattgcgtgcaaatttaccgaaattctggaaaaaattgatcgc

cgcaccggcaaaagcgtggaaaacaacccgaaatttattaaaagcggcgatgcggcgatt

gtgaaactgaccccgagcaaaccgatgtgcgtggaagcgtttaccgattatccgccgctg

ggccgctttgcggtgcgcgatatgcgccagaccgtggcggtgggcgtgattaaaagcgtg

gaaaaagcgaccgcgggcgcgggcaaagtgaccaaaagcgcggcgaaagcggcgaaaaaa

= 1380 nucleotides

D)

Amino acids = 460

E) Prokaryotic