3. What is the common label used to detect amplicons if you use agarose gel electrophoresis?...

3. What is the common label used to detect amplicons if you use agarose gel electrophoresis?

5. I ordered an 18-mer oligonucleotide primer from Fisher Scientific. What is an 18-mer?

8. You begin a PCR with 20 copies of the DNA template. After 5 cycles, approximately how many copies of amplicon do you have?

Expert Solution

3.

A common label used to detect DNA separated by Agarose Gel Electrophoresis is Ethidium Bromide (EtBr).

EtBr is an intercalating agent, which binds to the hydrophobic region of the DNA Nitrogenous bases by sandwiching between adjacent bases. When seen under UV light, EtBr fluoresces, which can be detected to 'visualize' DNA on an Agarose Gel.

4.

The suffix -mer is used to designate the length of DNA sequences. An 18-mer sequence has 18 nucleotides. The -mer suffix comes from the Greek Meros, or Part and is commonly used in Chemistry to denote the number of repeats in a structure. Aside from nucleotides, proteins are often referred to as monomers, dimers or trimers, which also has the same meaning. A DNA molecule that has n nucleotides, is an n-mer.

8.

Since there were 20 copies originally, each round doubles the number of copies in the previous round of replication.

Number of copies after round 1 = 20 * 2 = 40
Number of copies after round 2 = 40 * 2 = 80
Number of copies after round 3 = 80 * 2 = 160
Number of copies after round 4 = 160 * 2 = 320
Number of copies after round 5 = 320 * 2 = 640

Or,

Number of copies after 5 rounds, beginning with 20 copies = 20 * 2⁵ = 20 * 32 = 640

gladiator answered 9 months ago

What are the components of agarose gel electrophoresis set up?

What is the purpose of 1% agarose gel electrophoresis? Why is TAE buffer used during this...

What is the purpose of 1% agarose gel electrophoresis? Why is TAE buffer used during this procedure?

Linda has asked Brenn to prepare a 1% agarose gel for a gel electrophoresis analysis. But...

Linda has asked Brenn to prepare a 1% agarose gel for a gel electrophoresis analysis. But before he could do that, he needs to prepare 600 ml of 1x TAE from 95x TAE. How much 95x TAE and water should he add to create the 1x TAE solution? Afterwards, how much agarose does he need to weigh out, in grams, and add into 50 ml of 1x TAE?

In agarose gel electrophoresis of a sample of DNA, A) small DNA fragments in the sample...

In agarose gel electrophoresis of a sample of DNA, A) small DNA fragments in the sample will move more rapidly through the gel than large DNA fragments. B) the DNA will move through the gel toward the positive electrode. C) the higher the concentration of agarose in the gel, the more rapidly will the DNA move through the gel. D) A and B E) all of the above

Compare and contrast protein (SDS-PAGE) and DNA (agarose) gel electrophoresis.

When given the basis of the genetic test, analyse an agarose gel electrophoresis result to genotype...

When given the basis of the genetic test, analyse an agarose gel electrophoresis result to genotype individuals or identify mutations.

Compare and contrast native gel electrophoresis and denaturing gel electrophoresis. Which method would be used to...

Compare and contrast native gel electrophoresis and denaturing gel electrophoresis. Which method would be used to determine the molecular weight of a protein, why?

What are the limitations of using an agarose gel to determine if you are homozygous or...

What are the limitations of using an agarose gel to determine if you are homozygous or heterozygous with respect to the D1S80 locus?

1. List the arrangement of serum proteins from anode to cathode following agarose gel electrophoresis. 2....

1. List the arrangement of serum proteins from anode to cathode following agarose gel electrophoresis. 2. State one cause of each the following problems: no migration of proteins,tailing of bands, thin bands, “blobby” patterns.

Lab 9 – Molecular Biology In this lab, you will prepare an agarose gel and use...

Lab 9 – Molecular Biology In this lab, you will prepare an agarose gel and use gel electrophoresis to compare the size of 2 dye molecules Methyl orange and Ponceau G. You will also analyze an “unknown” sample that contains a mixture of two dyes. Dye molecules with lower molecular weight or greater electrical charge will migrate faster through the gel, than dye molecules with greater molecular weights or lesser electrical charge. Materials: Mini gel electrophoresis chamber 6 Tooth comb...

Question