In agarose gel electrophoresis of a sample of DNA,
A) small DNA fragments in the sample will move more rapidly
through the gel than large DNA fragments.
B) the DNA will move through the gel toward the positive
electrode.
C) the higher the concentration of agarose in the gel, the more
rapidly will the DNA move through the gel.
D) A and B
E) all of the above
3. What is the common label used to detect amplicons if you use
agarose gel electrophoresis?
5. I ordered an 18-mer oligonucleotide primer from Fisher
Scientific. What is an 18-mer?
8. You begin a PCR with 20 copies of the DNA template. After 5
cycles, approximately how many copies of amplicon do you have?
1. List the arrangement of serum proteins from anode to cathode
following agarose gel electrophoresis.
2. State one cause of each the following problems: no migration
of proteins,tailing of bands, thin bands, “blobby” patterns.
When you separate a DNA product of a restriction digest
on an agarose gel, and you see three bands on the gel spread out
between the negative and positive poles, what can you say about the
DNA, specifically the sizes of the DNA?
Completing the table below
PCR and gel electrophoresis
Key scientific principles which form the basis of the
technique:
PCR is a cyclic reaction that replicates specific DNA fragments
in vitro using the principle of double-stranded DNA replication. A
large number of copies of a specific DNA region can be amplified
rapidly by thermal cycling. DNA is visualised using gel
electrophoresis, fragments are pulled through a gel matrix by an
electric current and separated according to sizes. DNA ladder is
included...