Which type of enzyme is routinely used in biotechnology to cut DNA into fragments? Nuclear transplantation...

Which type of enzyme is routinely used in biotechnology to cut DNA into fragments?

Nuclear transplantation (somatic cell nuclear transfer) is a technique that requires multiple steps. Which of the following steps is nota part of this technique?
a. production of an enucleated egg (egg with the nucleus removed)

b. de-differentiated (re-programmed) somatic cell

c. fusion of a sperm cell with an egg cell

d. fusion of a de-differentiated somatic cell with an enucleated egg

Expert Solution

Restriction endonuclease are DNA cutting enzyme. They cut the DNA into fragments. Each enzyme recognises one or few target sequences and cut the DNA at or near those sequences.

Option C ( fusion of sperm cell with an egg cell)

Reason: This process involve 2 different cell and they are female gamete ( ovum/egg/oocyte) and the second cell is the somatic cell ( cell of human body except gametes. Example liver cell, skin cell etc). The genetic material of the donor egg cell is removed and discarded, leaving it 'deprogrammed'. Only somatic cell and enucleated cell is left after this. These are then fused by inserting the somatic cell into the empty ovum. After being inserted into the cell, the somatic cell nucleus is reprogrammed by its host egg cell. The ovum now containing the nucleus of the somatic cell is stimulated with a shock and will begin to divide.

Therefore it is now clear that there us no fusion of the sprem cell with the egg cell in this process.

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gladiator answered 10 months ago

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