Question

The enzyme is studied in the presence of a different inhibitor (inhibitor B). In this case, two different concentrations of inhibitor are used. Data are as follows:

a) Determine the apparent Vmax at each inhibitor concentration.

b) Determine the apparent KM at each inhibitor concentration.

c) Estimate KI from these data. [Hint: Calculate the K_I at 3 mM and at 5 mM; and then take the average of the two K_{I }values.]

v[(mmol/L)min−1]
[S](mmol/L)	No inhibitor	3 mM inhibitor B	5 mM inhibitor B
1.25	1.72	1.25	1.01
1.67	2.04	1.54	1.26
2.50	2.63	2.00	1.72
5.00	3.33	2.86	2.56
10.00	4.17	3.70	3.49

Answer 1