The effect of an inhibitor on an enzyme was tested and the experiment gave the results...

The effect of an inhibitor on an enzyme was tested and the experiment gave the results below. Plot on Excel the data using a double-reciprocal plot (Lineweaver-Burke), determine K_m and V_max of the no inhibitor and each type of inhibitor and the type of inhibition that is occurring.

[S] µM V (µmol/min) V (µmol/min) V (µmol/min)

with 0.0 nM with 25 nM with 50 nM

Inhibitor Inhibitor Inhibitor

______ ___________ ___________ ___________

0.4 0.22 0.21 0.20

0.67 0.29 0.26 0.24

1.00 0.32 0.30 0.28

2.00 0.40 0.36 0.32

Expert Solution

queen_honey_blossom answered 2 years ago

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