What is the role of each of the following components of PCR? DNA lysate: Forward and...

What is the role of each of the following components of PCR?

DNA lysate:
Forward and reverse primer
Taq Polymerase:
Nucleotides (dNTPs):
Magnesium

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role of each of the following components of PCR?

DNA lysate: used as a template in PCR.it contains template DNA. from this template DNA new DNA can be synthesized.
Forward and reverse primer : The primers are the short nucleotide sequences from where DNA synthesis begins.because Taq DNA polymerase can add nucleotide only onto a preexisting 3'-OH group,it needs a primer, onto which it can add first nucleotide.Forward primer attaches to the start codon while reverse primer attaches to stop codon.
Taq Polymerase : The main important function of Taq DNA polymerase is to replicate the DNA.Taq DNA polymerase catalyzes the formation of DNA.this enzyme is used because it can withstand at higher temperature.it adds the dNTP at growing DNA.
Nucleotides (dNTPs): In Polymerase chain reaction Taq DNA polymerase synthesizes a new DNA strand during successive cycles of PCR amplification.dNTPs are the building blocks of the DNA.there are four dNTPs are used they are :dATP,dCTP, dGTP and dTTP.
Magnesium = The magnesium concentration is a crucial factor in PCR during DNA amplification.The enzyme which is used in PCR is taq polymerase which is inactive .magnesium acts as a catalyzer and influences productivity.it acts as a cofactor for taq polymerase.

gladiator answered 1 year ago

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