In: Biology
1. How do each of the components in PCR work to replicate DNA
during a PCR reaction?
2. Why does the WT allele generate a different amplicon from that
of the mutant allele?
3. There are two sets of primers: one set to amplify the white
gene; another set that amplifies an unrelated gene. What are the
two sets for? What are the expected amplicon sizes for each
set?