1. How do each of the components in PCR work to replicate DNA during a PCR...

1. How do each of the components in PCR work to replicate DNA during a PCR reaction?

2. Why does the WT allele generate a different amplicon from that of the mutant allele?

3. There are two sets of primers: one set to amplify the white gene; another set that amplifies an unrelated gene. What are the two sets for? What are the expected amplicon sizes for each set?

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gladiator answered 1 year ago

Genetics Ch 10 How does PCR replicate DNA?

As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is...

As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is based on the way DNA is replicated in cells. During PCR template DNA plus other ingredients are placed in a test tube and the solution goes through repeated cycles of heating and cooling. The 'ingredients' of a basic PCR reaction are: DNA template, primer, nucleotides, DNA polymerase, and any salts required for polymerase function (buffer). No ligase is required in PCR but it is...

What is the role of each of the following components of PCR? DNA lysate: Forward and...

What is the role of each of the following components of PCR? DNA lysate: Forward and reverse primer Taq Polymerase: Nucleotides (dNTPs): Magnesium

How and where do many DNA viruses such as SV40 replicate in the host cell?

PCR takes advantage of the steps of DNA replication. Match each step of PCR with the...

PCR takes advantage of the steps of DNA replication. Match each step of PCR with the protein that the PCR step has replaced.

Explain how PCR (polymerase chain reaction) works. After explaining PCR, compare PCR to the DNA replication...

Explain how PCR (polymerase chain reaction) works. After explaining PCR, compare PCR to the DNA replication that occurs naturally in living cells, making sure to give at least 4 similarities and 4 differences.

what does the polymerase chain reaction (PCR) accomplish? 1) PCR converts RNA to DNA 2) PCR...

what does the polymerase chain reaction (PCR) accomplish? 1) PCR converts RNA to DNA 2) PCR corrects mutation in a gene 3) PCR makes many copies of DNA segment 4) PCR joins short segments of DNA to make one long segment how does the ribosome select the next amino acid to add to a growing polypeptide chain? 1) the ribosome selects the amino acid that can form hydrogen bonds with the mRNA 2) the ribosome selects a tRNA that base-pairs...

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1. How do each of the components in PCR work to replicate DNA during a PCR...

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