How to get the PI of a polypeptide.

Expert Solution

We start from the extreme right i.e., Glu and start building to the left

COOH of amino acid (Glu) is protected on solid support and NH₂ part is kept to form peptide bond with other amino acid (Lys) having NH₂ group protected and COOH part activated. The same process is repeated until the entire polypeptide is synthesized.

This can be understood with the help of the illustration below.

In the last step, solid support is removed to obtain the desired polypeptide.

queen_honey_blossom answered 2 years ago

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