Question

In: Chemistry

How will I find the pI for a non-ionizable amino acid?

How will I find the pI for a non-ionizable amino acid?

Solutions

Expert Solution

First, we need to know what is meant by pI.

pI refers to the isoelectric point of an amino acid. Isoelectric point of an amino acid is the pH at which the amino acid does not migrate in an electric field, i.e the pH at which it is neutral in charge.

Now, we need to differentiate between ionizable and non-ionizable amino acids while calculating iso-electric point. Ionizable amino acids are those which have readily ionizable side chain groups attached to it. These ionizable side chains can readily undergo proton exchange with the medium or with any other molecule. Thus the pKa value of each side chain group determines the pH value at which there will be neutral charge on the amino acid, i.e it determines its isoelectric point.

Out of the 20 most common naturally occuring amino acids, 7 are usually considered ionizable. They are aspartic acid, glutamic acid, histidine, arginine, lysine, tyrosine and cysteine. The rest can all be considered as non-ionizable amino acids.

For non-ionizable amino acids, where the side chain does not contain any ionizable group, the pI is simply the average of the pKa values of the amine and the carboxylic acid functional groups contained in that amino acid.

To calculate the pI, the amino acid needs to be considered in its zwitter-ionic form at the isoelectric point.

The methodical calculation of pI is illustrated in the image.


Related Solutions

Why aren't all amino acid R groups ionizable?
Why aren't all amino acid R groups ionizable?
1) If the pI for a particular amino acid is 7.5, at which pH will the...
1) If the pI for a particular amino acid is 7.5, at which pH will the net charge on the molecule be zero? 2) Calculate the pH of 0.00756 M HNO3. 3) The isoelectric point of an amino acid is defined as: a. the pH at which it exists in the basic form b. the pH equals the pKa c. the pH at which it exists in the acidic form d. the pH at which the amino acid exits in...
A nonstandard amino acid has three ionizable groups, with pKa values of 2.6, 7.4, and 10.13....
A nonstandard amino acid has three ionizable groups, with pKa values of 2.6, 7.4, and 10.13. A 0.1 M solution of this amino acid is made, 100 ml total, at pH 2.6. To this solution is added 10 ml of 1 M NaOH. What is the final pH of this solution after the addition? A) 3.2 B) 8.0 C) 7.4 D) 6.8 E) 10.13
describe the sources non essential amino acids in human organism.( for each non essential amino acid...please...
describe the sources non essential amino acids in human organism.( for each non essential amino acid...please more explanation)
What is an amino acid? How many amino acids are there? 
What is an amino acid? How many amino acids are there? 
All amino acids have two ionizable functional groups: an α-amino group (average pKa of 9.4) and...
All amino acids have two ionizable functional groups: an α-amino group (average pKa of 9.4) and an α-carboxylic acid group (average pKa of 2.2). Arginine also has an ionizable side chain (R group) with a pKa of ~12.5. One of the possible ionization states of arginine is shown below. At what pH would the above structure be the predominant ionization state? Note: Consider the ionization state of all three of the functional groups. The protonated form of the R group...
How do you find the pH of any amino acid at a particular pH. Please explain...
How do you find the pH of any amino acid at a particular pH. Please explain the basics clearly. I am completely lost. Thanks
The amino acid glutamic acid has the molecular formula C5H9NO4, and the amino acid isoleucine has...
The amino acid glutamic acid has the molecular formula C5H9NO4, and the amino acid isoleucine has the molecular formula C6H13NO2. Determine how many moles of isoleucine contain the same number of atoms as 1.96 mol of glutamic acid.
Write the net biochemical equation for the biosynthesis of the non-essential amino acid aspartate from pyruvate,...
Write the net biochemical equation for the biosynthesis of the non-essential amino acid aspartate from pyruvate, carbon dioxide and ammonia from the necessary elementary reactions or steps. (you must include all necessary elementary steps for full credit)
Write the net biochemical equation for the biosynthesis of the non-essential amino acid aspartate from pyruvate,...
Write the net biochemical equation for the biosynthesis of the non-essential amino acid aspartate from pyruvate, carbon dioxide and ammonia from the necessary elementary reactions or steps. (you must include all necessary elementary steps for full credit)
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT