Genetics Ch 10 How does PCR replicate DNA?

Genetics Ch 10

How does PCR replicate DNA?

Expert Solution

gladiator answered 2 years ago

As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is...

As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is based on the way DNA is replicated in cells. During PCR template DNA plus other ingredients are placed in a test tube and the solution goes through repeated cycles of heating and cooling. The 'ingredients' of a basic PCR reaction are: DNA template, primer, nucleotides, DNA polymerase, and any salts required for polymerase function (buffer). No ligase is required in PCR but it is...

1. How do each of the components in PCR work to replicate DNA during a PCR...

1. How do each of the components in PCR work to replicate DNA during a PCR reaction? 2. Why does the WT allele generate a different amplicon from that of the mutant allele? 3. There are two sets of primers: one set to amplify the white gene; another set that amplifies an unrelated gene. What are the two sets for? What are the expected amplicon sizes for each set?

Question of medical biology and genetics: What is PCR (polymerase chain reaction)? How does PCR work?...

Question of medical biology and genetics: What is PCR (polymerase chain reaction)? How does PCR work? What happens at each of pcr? Explain in details.

what does the polymerase chain reaction (PCR) accomplish? 1) PCR converts RNA to DNA 2) PCR...

what does the polymerase chain reaction (PCR) accomplish? 1) PCR converts RNA to DNA 2) PCR corrects mutation in a gene 3) PCR makes many copies of DNA segment 4) PCR joins short segments of DNA to make one long segment how does the ribosome select the next amino acid to add to a growing polypeptide chain? 1) the ribosome selects the amino acid that can form hydrogen bonds with the mRNA 2) the ribosome selects a tRNA that base-pairs...

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Explain how PCR (polymerase chain reaction) works. After explaining PCR, compare PCR to the DNA replication...

Explain how PCR (polymerase chain reaction) works. After explaining PCR, compare PCR to the DNA replication that occurs naturally in living cells, making sure to give at least 4 similarities and 4 differences.

Biological Anthropology: Ch. 3- Genetics: Reproducing Life & Producing Variation Ch. 4-Genes Their Evolution: Population Genetics...

Biological Anthropology: Ch. 3- Genetics: Reproducing Life & Producing Variation Ch. 4-Genes Their Evolution: Population Genetics (Please TYpe) Chapter 4 1. Microevolution, macroevolution 2. Reproductive isolation 3. Hardy-Weinberg Law 4. Four forces of evolution 5. Mutation types 6. Three patterns of natural selection 7. Admixture, founder’s effect Chapter 5 1. Race: historical and modern concepts, issues with the concept, etc. 2. Blumenbach, Boas, Lewontin 3. Four levels of human adaptation 4. Terms related to adaptation (stress, homeostasis, plasticity, functional adaptation)...

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1. If you started a PCR protocol with 10 double-stranded molecules of DNA, how many double-stranded...

1. If you started a PCR protocol with 10 double-stranded molecules of DNA, how many double-stranded molecules of the product would you have after 5 cycles of PCR? 2. What is the purpose of using DNA polymerase from Thermus aquaticus for the polymerase chain reaction rather than a DNA polymerase from a better characterized bacterium such as E. coli?

How are thermostable DNA Polymerases important for Polymerase Chain Reactions (PCR)?

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