If a histidine sidechain in a binding site is protonated in the free protein, but deprotonated...

If a histidine sidechain in a binding site is protonated in the free protein, but deprotonated in the ligand-bound state, how would pH affect the interaction?

A.) No effect

B.) Lower Kd at lower pH

C.) Higher Kd at lower pH

D.) Only ΔH would change with pH

Expert Solution

queen_honey_blossom answered 1 year ago

A protonated histidine residue in the active site of aspartate transcarbamoylase, ATCase, is thought to be...

A protonated histidine residue in the active site of aspartate transcarbamoylase, ATCase, is thought to be important in stabilizing the transition state of the bound substrate. A) Sketch a graph showing the pH dependence of the catalytic rate, assuming that this interaction is essential and dominates the pH-activity profile of the enzyme. Provide the biochemical basis for your graph. B) The ATCase mechanism is known to proceed via an ordered mechanism. Draw a Cleland notation diagram showing how this reaction...

What charge does a protonated amino group have? Deprotonated? What charge does a protonated carboxylic acid...

What charge does a protonated amino group have? Deprotonated? What charge does a protonated carboxylic acid have? Deprotonated?

How would a mutation in the lac repressor protein that was missing the effector binding site...

How would a mutation in the lac repressor protein that was missing the effector binding site behave in E. coli in the presence and absence of lactose? Would it produce B=-galactosidase?

How is Histidine used to modulate protein activity?

Draw the structure of protonated histidine and indicate (draw and name) all possible non bonding interactions...

Draw the structure of protonated histidine and indicate (draw and name) all possible non bonding interactions with water. include H bonding, and Hydration Spheres

1a. The term “transcription factor binding site” (aka TF binding site) refers to __

1a. The term “transcription factor binding site” (aka TF binding site) refers to __1b. Transcription factor binding sites are often found where within genomic DNA?1c. Transcription factor binding sites could be referred to as cis-acting elements because __1d. Transcription factors (both activators and repressors) could also be referred to as trans-acting factors because __ Terms Lac operon Operator Repressor protein CAP (catabolite activator protein) Trans acting factors vs. Cis acting elements Transcription factor Transcriptional activator vs. repressor DNA-binding domain Transcription factor binding site Activation vs. Repression domains Chromatin Nucleosome 10nm- vs. 30nm-fibers Euchromatin vs. heterochromatin Histones...

explain the distinction between binding site and binding region

Draw an IgG molecule and identify the following parts: Antigen-binding site Complement-binding region Macrophage-binding site Variable...

Draw an IgG molecule and identify the following parts: Antigen-binding site Complement-binding region Macrophage-binding site Variable region Hinge region

Discuss protein binding, what it means, what are the implications of a drug protein binding percentage...

Discuss protein binding, what it means, what are the implications of a drug protein binding percentage and how the protein binding impacts how drugs may interact with each other and the subsequent effect on the patient (provide 2 explicit examples). And, then explain what will happen if the patient is malnourished. what are ED50 , LD50, TD50: and explain in detail why the matter. Please give 2 examples of each.

What features of the binding site hinder oxygen binding to Hb? What feature of the binding...

What features of the binding site hinder oxygen binding to Hb? What feature of the binding site facilitates oxygen binding to Hb? Explain the Bohr effect using the theory of allosteric modulation of oxygen binding to hemoglobin.

Question