Describe the principle of separation in a column used for size –
exclusion chromatography. What is...
Describe the principle of separation in a column used for size –
exclusion chromatography. What is the separation range in term of
molecular weight? Give the definition of “gel filtration: and “gel
permeation”.
Size exclusion chromatography (SEC) is a technique that allows
for separation of molecules based on their size. If organic
solvents are used to separate polymers, this application is
referred to as gel permeation chromatography, and when aqueous
solvents are used to separate proteins and soluble macromolecules,
this is known as:
a.) Titration
b.) Paper chromatography
c.) P60 chromatography
d.) Silica gel column chromatography
e.) Gel filtration chromatography
State which separation technique (Gas Chromatography, Liquid
Chromatography, Electrophoresis, Size Exclusion Chromatography)
would be the best choice for each of the cases below and explain
WHY? thank you!
a. Separation of volatile components found in a perfume and that
result in its characteristic fragrance.
b. The determination of the molecular weight of a polymer that was
synthesized by a friend in a polymer synthesis lab.
Fall 2017
c. The separation of a nonvolatile pollutant found in drinking
water.
d. The...
State which separation technique (Gas Chromatography, Liquid
Chromatography, Electrophoresis, Size Exclusion Chromatography)
would be the best choice for each of the cases below and explain
WHY? thank you!
a. Separation of volatile components found in a perfume and that
result in its characteristic fragrance.
b. The determination of the molecular weight of a polymer that was
synthesized by a friend in a polymer synthesis lab.
Fall 2017
c. The separation of a nonvolatile pollutant found in drinking
water.
d. The...
How would you combine affinity chromotography together with size
exclusion chromatography to improve separation?
In the equilibrate step of the affinity chromotography protocol,
you accidentally use the elution buffer instead of the affinity
binding buffer. How will this affect the purification process?
Compare and contrast Native -PAGE, SDS-PAGE, size-exclusion
chromatography, and affinity chromatography, ion exchange
chromatography. How are components separated by these procedures
and how are they detected? Explain details. hHow are each of these
techniques carried out?
elect all statements below that apply to Column
Chromatography.
Column chromatography can be used to purify small (< 0.1 g)
sample mixtures.
The compound in a mixture with the lowest Rf value will exit the
column first.
Column chromatography is often used to purify large (10 g)
sample mixtures.
Column chromatography is used mainly as an analytical tool.
Column chromatography is useful in the separation of compounds.
Using the theory behind the technique, explain how it works. Why do
you think fluorene and 9-fluorenone where are the components of the
mixture you attempted to separate?