Experiment IV: Effect of pH on the Rate of Enzyme Reaction 3. What could be done...

Experiment IV: Effect of pH on the Rate of Enzyme Reaction

3. What could be done to more specifically determine the optimal pH?

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gladiator answered 1 year ago

Experiment IV: Effect of pH on the Rate of Enzyme Reaction 6. In this experiment, was...

Experiment IV: Effect of pH on the Rate of Enzyme Reaction 6. In this experiment, was the enzyme denatured or just slowed down at any of the pH values tested? What is the evidence? Materials for Experiment IV Five test tubes Test tube rack Disposable pipettes Potato extract Deionized water Water bath, 37 degrees Celsius (°C) 0.1 molar (M) sodium hydroxide (NaOH) 0.1 M hydrochloric acid (HCl) pH paper Procedure for Experiment IV Five test tubes were taken and labeled...

To the nearest hundredths, what is the pH at the end of an enzyme-catalyzed reaction if...

To the nearest hundredths, what is the pH at the end of an enzyme-catalyzed reaction if it were carried out in a 0.1 M buffer initially at pH 6.65, and 0.006 M of acid (H+) was produced during the reaction? (The buffer used was a polyprotic acid of the general form: H3A; the three pKas of this polyprotic acid are 2.11, 6.65, and 11.33.)

To the nearest hundredths, what is the pH at the end of an enzyme-catalyzed reaction if...

To the nearest hundredths, what is the pH at the end of an enzyme-catalyzed reaction if it were carried out in a 0.1 M buffer initially at pH 6.73, and 0.004 M of acid (H+) was produced during the reaction? (The buffer used was a polyprotic acid of the general form: H3A; the three pKas of this polyprotic acid are 2.17, 6.73, and 11.27.)

To the nearest hundredths, what is the pH at the end of an enzyme-catalyzed reaction if...

To the nearest hundredths, what is the pH at the end of an enzyme-catalyzed reaction if it were carried out in a 0.1 M buffer initially at pH 6.73, and 0.004 M of acid (H+) was produced during the reaction? (The buffer used was a polyprotic acid of the general form: H3A; the three pKas of this polyprotic acid are 2.17, 6.73, and 11.27.)

The effect of an inhibitor on an enzyme was tested and the experiment gave the results...

The effect of an inhibitor on an enzyme was tested and the experiment gave the results below. Plot on Excel the data using a double-reciprocal plot (Lineweaver-Burke), determine Km and Vmax of the no inhibitor and each type of inhibitor and the type of inhibition that is occurring. [S] µM V (µmol/min) V (µmol/min) V (µmol/min) with 0.0 nM with 25 nM with 50 nM Inhibitor Inhibitor Inhibitor ______ ___________ ___________ ___________ 0.4 0.22 0.21 0.20...

What kind of an effect does an enzyme have on the rate of the forward and...

What kind of an effect does an enzyme have on the rate of the forward and reverse reactions, the energy of the products and reactants and the energy of the transition state?

1. (3%) You measure the initial rate of an enzyme reaction as a function of substrate...

1. (3%) You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data are obtained: [S] V0 –Inhibitor +Inhibitor 0.0001 33 17 0.0002 50 29 0.0005 71 50 0.001 83 67 0.002 91 80 0.005 96 91 0.01 98 95 0.02 99 98 0.05 100 99 0.1 100 100 0.2 100 100 a) What is the Vmax in the absence of inhibitor? b) What is...

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Experiment IV: Effect of pH on the Rate of Enzyme Reaction 3. What could be done...

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