Question

What do people consider is the weakest and strongest aspects of doing the API for finding the unknown bacteria?

Answer 1

ANS :The analytical profile index or API is a classification of bacteria based on experiments, allowing fast identification. This system is developed for quick identification of clinically relevant bacteria. Because of this, only known bacteria can be identified.

A dichotomous key is a common tool used by biologists and other naturalists to categorize an unknown organism. This “key” is a series of questions or statements asking about a specific characteristic of the organism.

The reasons for accurate identification of pathogenic bacteria are:

1. Interpretation of antibiotic susceptibility testing often rests on identification. Two different bacteria may give the same values on this test, but the definitions of susceptible and resistant are different in different species.
2. Some species can be notoriously difficult to eradicate by treatment, and require long term use of antibiotics. Without identifying the pathogen, you can’t predict this.
3. Some species are intrinsically resistant to certain antibiotics. In such isolates, even if the susceptibility testing results suggest that the organism is sensitive to the antibiotic in question, that antibiotic will be useless in treatment. So, certain antibiotics are automatically reported as resistant in such cases. Without knowing the identity of the isolate, such predictions are impossible.