2. How would you produce a 10-1 dilution of a 3 ml sample using the entire...

2. How would you produce a 10^-1 dilution of a 3 ml sample using the entire 3 ml volume?

3. What is the dilution if 96 ml of diluent is added to 4 ml of bacterial suspension?

4. Plating 1.0 ml of a sample diluted by a factor of 10^-3 produced 43 colonies. What was the original cell density in the sample?

5. Plating 0.1 ml of a sample diluted by a factor of 10^-3 produced 43 colonies. What was the original cell density in the sample?

Expert Solution

Answers to all the questions are given below in handwritten.Please find the attached:

gladiator answered 3 years ago

1. If you had a 1 ml liquid culture, how would you make a 1:10 dilution?...

1. If you had a 1 ml liquid culture, how would you make a 1:10 dilution? A 1:50 dilution? A 1:100 dilution? 2. If you make a 1:100 dilution with a final volume of 1 ml, then use 100 ul or 0.1 ml into 900 ul of H2O. What would be your final dilution?

if 0.1 mL of a 10^-2 dilution is spread on a agar plate, what dilution will...

if 0.1 mL of a 10^-2 dilution is spread on a agar plate, what dilution will it be considered when calculating the original number of cells in the sample?

You counted 620 colonies on a plate with a dilution of 10-2. How many cells/ml were...

You counted 620 colonies on a plate with a dilution of 10-2. How many cells/ml were in the original sample?

A 75 mL sample of water was placed in a 300 mL BOD bottle, and dilution...

A 75 mL sample of water was placed in a 300 mL BOD bottle, and dilution water was added to fill the bottle. The initial DO concentration was 8.28 mg/L. The DO reading after 5 days was 4.56 mg/L and after 8 days was 3.59 mg/L. Determine the reaction constant (k) - a trial-and-error solution needed Determine BODu

In 2-3 sentences, describe howto do a serial dilution of a 1x 10-3Msolution to obtain 1...

In 2-3 sentences, describe howto do a serial dilution of a 1x 10-3Msolution to obtain 1 L of a 1x 10-9M solution. (Include necessary volumes and equipment.)

The dilution used for plating into spread plate was 10^-2 What is the dilution factor to...

The dilution used for plating into spread plate was 10^-2 What is the dilution factor to be used in the formula (CFU x Dilution factor)/plating factor

solution, mL dissolved rock 1. 10 2. 10 3. &nb

solution, mL dissolved rock 1. 10 2. 10 3. 10 4. 10 5. 10 mL 0.100M sodium metasilicate added 0 1.00 2.00 4.00 20.00 total volume 50 50 50 50 50 Si Signal (mA) 0.235 0.500 0.768 1.250 5.202 A 10.000g sample of rock containing quartz as the sole source of silicon is dissolved and diluted to 1.000 +/- 0.010 liters and analyzed by the method of standard additions. What is the wt% of quartz in the rock?

What is the final dilution of a liquid diluted first by 10^-1, then 10^-2, again at...

What is the final dilution of a liquid diluted first by 10^-1, then 10^-2, again at 10^-2, and finally 10^-1?

Five ul of a 10-to-1 dilution of a sample were added to 3ml of Bradford reagent....

Five ul of a 10-to-1 dilution of a sample were added to 3ml of Bradford reagent. The absorbance at 595 nm was 0.78 and, according to a standard curve, correpsonds to 0.015mg of protein on the x axis. What is the protein concentration of the original solution?

How to find theoretical yield using 6 mL 1-propanol, 10 mL acetic acid and 1 mL...

How to find theoretical yield using 6 mL 1-propanol, 10 mL acetic acid and 1 mL sulfuric acid.

Question