In: Chemistry
You are going to use column chromatography to separate a mixture
of four dyes: 4-phenylazophenol, Scarlet-R, Sudan II,
4-phenylazoanisole.
a. Which compound would you expect to elute from the column first
and which one last? Explain.
b. What solvent system will be used to elute these compounds from
the column?
In traditional column chromatography, the compounds which have polar groups in their structures will have more affinity towards stationary phase (for example silica gel) and these compounds elute last in the column chromatography.
Compounds which are not having polar groups will not have any interactions with the stationary phase and these compounds elute first from the column.